247 pcmv pe2 p2a gfp (Addgene inc)
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247 pcmv pe2 p2a gfp
247 Pcmv Pe2 P2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+pe2+p2a+gfp/pCMV-PE2-P2A-GFP+(Plasmid+%23132776)/10__1158_slash_0008___5472__can___25___2893-94-9-11
Average 95 stars, based on 51 article reviews
247 Pcmv Pe2 P2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+pe2+p2a+gfp/pCMV-PE2-P2A-GFP+(Plasmid+%23132776)/10__1158_slash_0008___5472__can___25___2893-94-9-11
Average 95 stars, based on 51 article reviews
247 pcmv pe2 p2a gfp - by Bioz Stars,
2026-10
95/100 stars
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Plasmid Preparation:Article Title: Development of HEK293T cell reference materials for β-thalassemia genetic testing using prime editing Article Snippet: .. Article Title: A novel human hepatocyte cell line to study PNPLA3-associated steatotic liver disease. Article Snippet: 5 Hepatogenomics Research Group, School of Biomedical Sciences, Queensland 6 University of Technology (QUT), Brisbane, Queensland, Australia; Metallogenomics 7 Laboratory, School of Biomedical Sciences, Queensland University of Technology 8 (QUT), Brisbane, Queensland, Australia; Centre for Genomics and Personalised 9 Health, Queensland University of Technology (QUT), Brisbane, Queensland, Australia 10 11 12 13 Running head: A novel PNPLA3 p.I148M model of steatotic liver disease 14 15 16 17 18 19 20 21 22 23 Corresponding author: 24 Professor V. Nathan Subramaniam 25 Hepatogenomics Research Group 26 Centre for Genomics and Personalised Health 27 School of Biomedical Sciences, Queensland University of Technology (QUT) 28 60 Musk Ave, Kelvin Grove, Queensland 4059, Australia.. 29 Tel: +617 3138 2980; Fax: +617 3138 6030 30 E-mail: nathan.subramaniam@qut.edu.au 31 Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage under autophagy-deficient conditions. Article Snippet: .. A B 0 10 20 30 Li ve r w ei gh t/B od y w ei gh t ( % ) p = 5.50e-14 p = 2.95e-11 p = 5.50e-14 p = 3.72e-10 p = 1.40e-9 0 5 10 15 20 25 Li ve r w ei gh t/B od y w ei gh t ( % ) p = 4.57e-10 p = 6.89e-11 p = 4.54e-12 0 50 100 150 Ab er ra nt a re a (% ) p = 3.01e-12 p = 7.45e-11 p = 7.77e-12 p = 2.07e-10 p = 0.0155 Ab er ra nt a re a (% ) 0 20 40 60 80 100 p = 2.07e-6 p = 1.11e-6 p = 8.22e-6 0 1 2 3 4 C K1 9 po si tiv e ar ea (% ) p = 0.00137 p = 7.10e-4 p = 6.30e-4 C D E F 0.0 0.5 1.0 1.5 2.0 2.5 C K1 9 po si tiv e ar ea (% ) p = 4.12e-3 p = 1.66e-4 p = 0.0244 p = 0.00102 cont. p62S351A Atg7-KO;p62S351A Atg7-KO cont. p62T352A Atg7-KO;p62T352A Atg7-KO cont. p62S351A Atg7-KO;p62S351A Atg7-KO cont. p62T352A Atg7-KO;p62T352A Atg7-KO cont. p62S351A Atg7-KO;p62S351A Atg7-KO cont. p62T352A Atg7-KO;p62T352A Atg7-KO cont. p62S351A Atg7-KO;p62S351AAtg7-KO cont. p62S351A Atg7-KO;p62S351AAtg7-KO cont. p62T352A Atg7-KO;p62T352AAtg7-KO cont. p62T352A Atg7-KO;p62T352AAtg7-KO 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on June 2, 2025 from IP 114.10.29.55. originally constructed using Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage in autophagy-deficient conditions Article Snippet: .. The CAG promoter-driven prime editor 2 (PE2) and the U6 promoter-driven pegRNA expression vectors were originally constructed using Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist. Article Snippet: Oligos were synthesized by Eurofins Genomics (Egersberg, Germany). .. PE plasmid 30 is Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage under autophagy-deficient conditions Article Snippet: .. The CAG promoter-driven prime editor 2 (PE2) and the U6 promoter-driven pegRNA expression vectors were originally constructed using Article Title: Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids Article Snippet: .. CRISPR:Article Title: A novel human hepatocyte cell line to study PNPLA3-associated steatotic liver disease. Article Snippet: 5 Hepatogenomics Research Group, School of Biomedical Sciences, Queensland 6 University of Technology (QUT), Brisbane, Queensland, Australia; Metallogenomics 7 Laboratory, School of Biomedical Sciences, Queensland University of Technology 8 (QUT), Brisbane, Queensland, Australia; Centre for Genomics and Personalised 9 Health, Queensland University of Technology (QUT), Brisbane, Queensland, Australia 10 11 12 13 Running head: A novel PNPLA3 p.I148M model of steatotic liver disease 14 15 16 17 18 19 20 21 22 23 Corresponding author: 24 Professor V. Nathan Subramaniam 25 Hepatogenomics Research Group 26 Centre for Genomics and Personalised Health 27 School of Biomedical Sciences, Queensland University of Technology (QUT) 28 60 Musk Ave, Kelvin Grove, Queensland 4059, Australia.. 29 Tel: +617 3138 2980; Fax: +617 3138 6030 30 E-mail: nathan.subramaniam@qut.edu.au 31 Sequencing:Article Title: Engineered methionine adenosyltransferase cascades for metabolic labeling of individual DNA methylomes in live cells Article Snippet: 10 μM of complementary single stranded pegRNA and sgRNA oligonucleotides (Metabion, Table S4) were phosphorylated with 5 U T4 PNK (ThermoFisher Scientific) in PNK buffer supplemented with 1 mM of ATP at 37 °C for 30 min, denatured at 95°C for 5 min and annealed at cooling rate of 0.1 °C /s. pegRNA sequences were cloned into pU6-pegRNA-GG-acceptor (Addgene #132777) incubating 2.5 nM of annealed oligonucleotides, 30 ng of vector, 5 U FastDigest Eco31I (ThermoFisher Scientific) and 5 U T4 DNA ligase (ThermoFisher Scientific) in ligase buffer at 37°C for 5 min followed by incubation at 22 °C for 5 min, repeating the reactions for 6 cycles. sgRNA coding vector was constructed cloning prepared oligonucleotides into phU6-gRNA (Addgene #53188) at BpiI sites following the same ligation conditions. .. Next, the puromycin coding sequence was amplified from px459 (Addgene #62988) and cloned into Amplification:Article Title: Engineered methionine adenosyltransferase cascades for metabolic labeling of individual DNA methylomes in live cells Article Snippet: 10 μM of complementary single stranded pegRNA and sgRNA oligonucleotides (Metabion, Table S4) were phosphorylated with 5 U T4 PNK (ThermoFisher Scientific) in PNK buffer supplemented with 1 mM of ATP at 37 °C for 30 min, denatured at 95°C for 5 min and annealed at cooling rate of 0.1 °C /s. pegRNA sequences were cloned into pU6-pegRNA-GG-acceptor (Addgene #132777) incubating 2.5 nM of annealed oligonucleotides, 30 ng of vector, 5 U FastDigest Eco31I (ThermoFisher Scientific) and 5 U T4 DNA ligase (ThermoFisher Scientific) in ligase buffer at 37°C for 5 min followed by incubation at 22 °C for 5 min, repeating the reactions for 6 cycles. sgRNA coding vector was constructed cloning prepared oligonucleotides into phU6-gRNA (Addgene #53188) at BpiI sites following the same ligation conditions. .. Next, the puromycin coding sequence was amplified from px459 (Addgene #62988) and cloned into Clone Assay:Article Title: Engineered methionine adenosyltransferase cascades for metabolic labeling of individual DNA methylomes in live cells Article Snippet: 10 μM of complementary single stranded pegRNA and sgRNA oligonucleotides (Metabion, Table S4) were phosphorylated with 5 U T4 PNK (ThermoFisher Scientific) in PNK buffer supplemented with 1 mM of ATP at 37 °C for 30 min, denatured at 95°C for 5 min and annealed at cooling rate of 0.1 °C /s. pegRNA sequences were cloned into pU6-pegRNA-GG-acceptor (Addgene #132777) incubating 2.5 nM of annealed oligonucleotides, 30 ng of vector, 5 U FastDigest Eco31I (ThermoFisher Scientific) and 5 U T4 DNA ligase (ThermoFisher Scientific) in ligase buffer at 37°C for 5 min followed by incubation at 22 °C for 5 min, repeating the reactions for 6 cycles. sgRNA coding vector was constructed cloning prepared oligonucleotides into phU6-gRNA (Addgene #53188) at BpiI sites following the same ligation conditions. .. Next, the puromycin coding sequence was amplified from px459 (Addgene #62988) and cloned into Construct:Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage under autophagy-deficient conditions. Article Snippet: .. A B 0 10 20 30 Li ve r w ei gh t/B od y w ei gh t ( % ) p = 5.50e-14 p = 2.95e-11 p = 5.50e-14 p = 3.72e-10 p = 1.40e-9 0 5 10 15 20 25 Li ve r w ei gh t/B od y w ei gh t ( % ) p = 4.57e-10 p = 6.89e-11 p = 4.54e-12 0 50 100 150 Ab er ra nt a re a (% ) p = 3.01e-12 p = 7.45e-11 p = 7.77e-12 p = 2.07e-10 p = 0.0155 Ab er ra nt a re a (% ) 0 20 40 60 80 100 p = 2.07e-6 p = 1.11e-6 p = 8.22e-6 0 1 2 3 4 C K1 9 po si tiv e ar ea (% ) p = 0.00137 p = 7.10e-4 p = 6.30e-4 C D E F 0.0 0.5 1.0 1.5 2.0 2.5 C K1 9 po si tiv e ar ea (% ) p = 4.12e-3 p = 1.66e-4 p = 0.0244 p = 0.00102 cont. p62S351A Atg7-KO;p62S351A Atg7-KO cont. p62T352A Atg7-KO;p62T352A Atg7-KO cont. p62S351A Atg7-KO;p62S351A Atg7-KO cont. p62T352A Atg7-KO;p62T352A Atg7-KO cont. p62S351A Atg7-KO;p62S351A Atg7-KO cont. p62T352A Atg7-KO;p62T352A Atg7-KO cont. p62S351A Atg7-KO;p62S351AAtg7-KO cont. p62S351A Atg7-KO;p62S351AAtg7-KO cont. p62T352A Atg7-KO;p62T352AAtg7-KO cont. p62T352A Atg7-KO;p62T352AAtg7-KO 14 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on June 2, 2025 from IP 114.10.29.55. originally constructed using Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage in autophagy-deficient conditions Article Snippet: .. The CAG promoter-driven prime editor 2 (PE2) and the U6 promoter-driven pegRNA expression vectors were originally constructed using Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage under autophagy-deficient conditions Article Snippet: .. The CAG promoter-driven prime editor 2 (PE2) and the U6 promoter-driven pegRNA expression vectors were originally constructed using Expressing:Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage in autophagy-deficient conditions Article Snippet: .. The CAG promoter-driven prime editor 2 (PE2) and the U6 promoter-driven pegRNA expression vectors were originally constructed using Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells - reversal by a GLP-1 receptor agonist. Article Snippet: Oligos were synthesized by Eurofins Genomics (Egersberg, Germany). .. PE plasmid 30 is Article Title: KEAP1 retention in phase-separated p62 bodies drives liver damage under autophagy-deficient conditions Article Snippet: .. The CAG promoter-driven prime editor 2 (PE2) and the U6 promoter-driven pegRNA expression vectors were originally constructed using |